Barcode High-Throughput Sequencing Service

Do you have relatively high numbers of PCR products or plasmids that can or need to be sequenced in parallel? Then we recommend our Barcode High-Throughput Sequencing Service (prepaid service type).


Main Service Features and Benefits:

  • Read length per run: up to 1100 bases
  • Rapid turnaround time: sequencing time in the laboratory down to 3 days
  • Direct access to support by experienced academic staff (no waiting on a recorded-message hotline)
  • A series of useful additional services just for FREE 
    • PCR purifications or plasmid preparations (from E. coli)
    • Standard but also up to 12 specific primers per plate can be added
    • Special treatment for GC-rich templates1
    • Possibility to fine-tune software analysis parameters to achieve your desired performance
    • Helpful online tools enabling you to oversee your stock of labels and primers but also to share it amongst your group members
    • Convenient sample pickup service; if not available for your location, you can use our pre-paid and pre-addressed envelopes for mail shipment
  • Further convenient services at an extra charge 
    • Overnight in-house production of specific sequencing primers
    • Clone picking into 96-well plates and generation of glycerol stocks
    • Return delivery of isolated plasmids2 
Sample Requirements
See our user guide under "Related Downloads".

Important – Please Note!
  • If you send us 96 samples to be sequenced with a forward as well as a reverse primer, please consider that you need to ship 2 prepaid labels along with your samples since the total amount of reactions is 192
  • In case you need to define more than one primer source (e.g. 72 samples shall be sequenced with a standard primer from Microsynth’s Standard Primer List whereas 24 samples require a specific sequencing primer from your Custom Primer List), please click on “Fill Order Form (Multiple Primer Sources)”.


How to Order?

a) How to order a batch of prepaid labels or plates:

  • Enter our webshop
  • Click on Plate Sequencing in the green DNA Sequencing area
  • Click on Order Prepaid Labels in the Barcode High-Throughput Service area and follow the further instructions

b) How to submit prepaid plates for sequencing:

  • Enter our webshop
  • Click on Select Services in the “DNA Sequencing” menu
  • Click on Fill Order Form in the Barcode High-Throughput Service area and follow the further instructions

1 You are recommended to choose this option in case your sequence contains GC-rich sequences or hairpin structures. We then apply a protocol which has been developed specifically for GC-rich sequencing templates. Routine samples show best results with the standard protocol, whereas GC-rich samples show best results with this specific protocol.
2 Condition: you send us high-copy plasmid(s) with only one antibiotic resistance.

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Write an e-mail or call us at +41-71-722 83 33

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