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Antisense Oligos

 
 
In antisense technology short synthetic oligonucleotides are supposed to hybridize to a certain sequence of the mRNA (drug target) thereby interfering with the mRNA processing. In order to equip an oligonucleotide with drug-like properties, chemical modification is inevitable. Microsynth provides most prominent modification strategies that are used in antisense technology like:
  • PTO (phosphorothioate)
  • 2'-O-Me-RNA
  • 2'-MOE-RNA
  • Gapmers
 

Features and Benefits

 
 
High Quality
  • all ASOs are MALDI-TOF MS controlled before shipment
  • Renowned ASO supplier for top research institutes and pharma/biotech companies

 

Fast Production
  • ASO oligos: ≤ 2 d
 
Excellent Technical Support
  • Trained scientists with molecular biology background are happy to support you (from 8 am to 5 pm)!
 
 
 
Cost Effective
  • Competitive pricing for repetitive as well as volume orders
  • High guaranteed yields and/or average delivery yields

 

Convenient
  • Analytical HPLC available
  • Certificate of Analysis available
  • Possibility to upgrade manufacturing process from research-use-only to preclinical use requirements
 
User-friendly Online Ordering System
  • New and easy-to-use online portal with a series of helpful tools (e.g. order tracking & history, convenient search and re-order option)
 

Overview

 

Microsynth can offer you the following three modifications:

  • PTO (phosphorothioates) modifications: PTOs contain one sulfur atom in place of an oxygen atom in the internucleotide linkage of DNA or RNA. This modification of the normal phosphodiester backbone is characterized by an increased cell uptake, high nuclease resistance and elicitation of RNAse H activity.
 
  • 2'-O-Me-RNA modifications: The incorporation of 2'-O-Methyl RNA nucleotides induces a resistance to a wide variety of nucleases, in particular RNase. Furthermore, 2’-OMe oligonucleotides show slightly increased affinity towards their complementary mRNA target sequence, thereby forming more stable hybrid duplexes compared to their non-modified DNA or RNA counterparts. This enables the formation of more stable hybrids with complementary RNA strands than would be the case for non-modified DNA and RNA sequences.
 
  • 2'-MOE-RNA modifications: Oligonucleotides incorporating 2'-O-methoxyethyl (MOE)-modified nucleotides, can support most, if not all antisense mechanisms of action. Further key distinctive characteristics are nuclease resistance, lower toxicity, superior target binding specificity, as well as increased affinity towards complementary RNA. For more detailed information about 2'-MOE antisense oligonucleotides from Microsynth, please see the flyer under "Related Downloads".

To learn more about specifications of these three types of modifications, please see the table below.


Key Specifications:

Modifications

Position

Synthesis scale [µmol] Purification1

5’

3’

Int.

0.04

0.2

1

15

LS2

HPLC + Dialysis

PTO

   

x

x

x

x

x

x

x

2’-O-Me-RNA

x

x

x

 

x

x

x

x

x

2'-MOE-RNA x x x   x x x x x
 
 

1 We strongly recommend to order ASOs with “HPLC + Dialysis” purification. The Na+ salt exchange is necessary to remove toxic salts from HPLC purification. The same yields apply as for unmodified standard oligos.

LS: abbreviation for large scale synthesis with synthesis yields up to gram amounts

How to Order

 
How to Order?
  • Enter our webshop
  • Click on DNA in the blue "DNA/RNA Synthesis" domain
  • Select either Normal Entry in order to type or copy/paste the desired sequence information etc. or alternatively select Upload Entry by using our convenient Excel template (can be downloaded from our webshop).
 
PTO modified oligonucleotides: please mark PTO bonds by inserting an asterisk between the bases, e.g. AAA*T*G*CCC*AAA.
 
 
2’-O-Me-RNA (Selective incorporation of 2’-O-Me-RNA bases into DNA):
  • Enter 5,6,7,8 for each distinct base to be replaced inside the sequence
  • Define Inner Modification (5=…) by selecting the desired modification from the drop down menu (e.g. 5=…2’-O-Methyl-RNA-A, 6=…2’-O-Methyl-RNA-C etc.)
 
2’-MOE-RNA (Selective incorporation of 2’-MOE-RNA bases into DNA):
  • Enter 5,6,7,8 for each distinct base to be replaced inside the sequence
  • Define Inner Modification (5=…) by selecting the desired modification from the drop down menu (e.g. 5=…2’-Methoxyethyl-ribo-A, 6=…2’-Methoxyethyl-ribo-mC etc.)