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Barcode High-Throughput Run

 
 
Best-in-Class Sanger sequencing service in 96-well plates for PCR products and plasmids. The Barcode High-Throughput Run includes plasmid isolation from E. coli or PCR purification. This prepaid service (per plate pricing) is very popular due to its excellent price-performance ratio.

Features and Benefits

 
 
High Quality
  • DNA sequencing read lengths up to 1’100 bases or more in Phred20 quality
  • Quality assurance statements ISO/IEC 17025:2005 (STS 0429)
 
Fast
  • Rapid turnaround time: sequencing time in the laboratory down to 1 day
  • Results are delivered Monday to Saturday
 
Convenient
  • Use our drop box in your vicinity for free shipping of samples
  • Possibility to fine-tune sequence software analysis parameters (e.g. IUPAC base calling, sequence trimming)
  • Up to 12 months storage for ordered and enclosed sequencing primers
  • 96-well plates and stripes are provided with the barcode labels
 
Environmentally-Friendly Sample Pick-Up
  • Your sequencing samples are predominantly shipped using environmentally-friendly transport vehicles (train, bicycle)
 
 
Cost-effective
  • Significant price advantage due to its prepayment modality
  • PCR purification and plasmid isolation from E.coli free of charge
  • Standard vector primers (>90) but also specific primers are added free of charge
  • Treatment for GC-rich templates and hairpin structures can be selected free of charge
 
User-Friendly Online Ordering System
  • Easy-to-use online shop with lots of useful tools
  • Primer management tool to define your customized primer list
  • Group management tool to share barcode labels and primers among group members
 
Technical Support
  • Direct access to support by experienced academic staff
  • Available at no charge to discuss your project, troubleshoot, and answer questions
  • Useful information for initial troubleshooting is provided automatically with sequencing results that are difficult to sequence
 
 

Additional Services

 
 

Following useful and complementary services can be purchased at a small extra charge1 to further upgrade Microsynth's Barcode High-Throughput Run:

  • On request isolated plasmids are sent back to customer's lab2
  • Colony picking from agar plates into high throughput 96-well plates and generation of glycerol stocks
  • Higher Scale plasmid isolation (Midi/Maxi) from selected clones on request

1  Complementary services can be chosen when placing a sequencing order at our online shop. You will receive an invoice covering the costs that are not included in the price for the prepaid barcode labels.

2  Condition: you send us high-copy plasmids. Only one antibiotic resistance can be used within the same plate.

How to Order

 

1. Procedure to order a batch of prepaid labels:

  • Enter our webshop
  • Click on Plate Sequencing in the green "DNA Sequencing" area
  • Click on Order Prepaid Labels under "Barcode High-Throughput Run" and follow the further instructions

2. Procedure to use our Barcode High-Throughput Run for sequencing:

  • Enter our webshop
  • Click on Plate Sequencing in the green "DNA Sequencing" area
  • Click on Fill Order Form under "Barcode High-Throughput Run" and follow the further instructions

 

See also Microsynth's user guide on the right side for more detailed information.