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CRSPR Editing

 
 
CRISPR/Cas9-mediated gene editing and related approaches enable the targeted genetic engineering of virtually any organism. Microsynth is experienced in the analyses of cell edits, either at the cell pool level or on expanded clones.
 

Editing Frequency

 
Microsynth offers to quantify InDel frequencies at CRISPR/Cas9 sites of edited cell pools, using a PCR strategy combined with Sanger sequencing; the services include the complete analytical procedure from DNA isolation to bioinformatics analyses if desired.
 
 

InDel Structure Analysis

 
The InDel structure at the bp level for CRISPR/Cas9 sites of edited cell pools can be quantified using an amplicon deep sequencing strategy. Microsynth offers to develop the assay, and to run the analytical procedure from DNA isolation to bioinformatics.