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Customized qPCR Assays

 
 
Fast and reliable development of custom and MIQE-compliant qPCR assays. Upon forwarding us your target sequence or accession number, Microsynth will deliver your assay within a 2-3 weeks time frame.
 

Features and Benefits

 
High Quality
  • Real-time PCR assays with proven functionality due to comprehensive testing using a specific positive control as well as a no template control
  • High sensitivity due to optimization of PCR working conditions: we design, synthesize and test your primer set(s) for their PCR performance

 

Cost Effective
  • Low-cost reordering of primer/probe sets, e.g. via our own oligonucleotide production facility (you will be provided with complete sequence of all oligonucleotides)
  • High quantity delivered: QC-tested primer/probe set(s) in single tubes (aliquoted) are sufficient for more than 2000 PCR reactions

 

 
Convenient
  • High degree of customization: you as our customer will decide

    • whether the assay should be singleplex or multiplex
    • which pair(s) of possible reporter dyes and quenchers should be selected
    • which sequence areas should be considered or not considered when designing the primer/probe set(s)
  • Easy publishing due to compliance with MIQE guidelines (full transparency with respect to  sequence information of used primer and probes)

 

Applications

 
  • Gene expression analysis in any organism
  • Gene quantification at genomic level
  • SNP (single nucleotide analysis) genotyping and analysis of polymorphisms

How to Order

 
To obtain information about prices or to place an order please contact us.