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Detection of Cow & Pig DNA in Anticoagulant

 

Pharmaceutical grade anticoagulant is derived from mucosal tissues of pig intestines or bovine lungs. Microsynth was inquired by an industrial customer to develop a sensitive multiplex real-time PCR assay, which is suitable to quantify residual traces of pig and cow DNA in anticoagulant and conduct routine testing on product samples. Since the anticoagulant is known to be a strong inhibitor in PCR/real-time PCR assays, a major challenge of the project was to develop a robust protocol, which includes the specific digestion of the anticoagulant.

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