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High-Throughput Run

Flexible Sanger sequencing service in plates for PCR products and plasmids. The High-Throughput Run is a non-prepaid service. The per reaction pricing makes it especially attractive for customers with medium sequencing demand, which do not always have a full 96-well plate. You can send plates starting from 24 samples per plate.

Features and Benefits

High Quality
  • DNA sequencing read lengths up to 1’100 bases or more in Phred20 quality
  • Quality assurance statements ISO/IEC 17025:2005 (STS 0429)


  • Rapid turnaround time: sequencing time in the laboratory down to 1 day
  • Results are delivered Monday to Friday
  • Plates with at least 24 samples can be sent, pricing by reaction
  • Use our drop box in your vicinity for free shipping of samples
  • Possibility to fine-tune sequence software analysis parameters (e.g. IUPAC base calling, sequence trimming)
  • Up to 12 months storage for ordered and enclosed sequencing primers
Environmentally-Friendly Sample Pick-Up
  • Your sequencing samples are predominantly shipped using environmentally-friendly transport vehicles (train, bicycle)
  • Standard vector primers (>90) but also specific primers are added free of charge
  • Optimized sequencing methods for GC-rich templates or hairpin structures free of charge


User-friendly Online Ordering System
  • Easy-to-use online shop with lots of useful tools
  • Primer management tool to define your customized primer list
  • Group management tool to share primers among group members
Technical Support
  • Direct access to support by experienced academic staff
  • Available at no charge to discuss your project, troubleshoot, and answer questions
  • Useful information for initial troubleshooting is provided automatically with sequencing results that are difficult to sequence

Additional Services

Following useful and complementary services can be purchased at a small extra charge to further upgrade Microsynth's High-Throughput Run:
  • Colony picking from agar plates into high throughput 96-well plates and generation of glycerol stocks
  • PCR purifications or plasmid preparations (from E. coli)
  • On request isolated plasmids are sent back to customers (up to transfection grade, scalable up to 10 mg). 
  • Project-based mutation reports
  • Sequencing of large DNA constructs such as BACs, cosmids and fosmids

How to order

Procedure to use our High-Throughput Run service for sequencing:
  • Enter our webshop
  • Click on Fill Order Form under "High-Throughput Run" and follow the further instructions
See also Microsynth's user guide on the right side for more detailed information.