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Primer Walking Service

 
 
Fast and comfortable Sanger sequencing service for DNA templates longer than 1'400 bases. By sequencing from both ends we advance about 1600 base pairs per day.

Overview

 
Microsynth's Primer Walking Service offers single-stranded (SS) and double-stranded (DS)1 sequencing of your DNA templates. Both options can be selected with and without sequence assembly. In case of DS assembled service, Microsynth offers sequence verification as an additional comfortable service (you just have to provide us your reference sequence).
 

 

1 In the double-stranded Primer Walking Service both strands of your DNA sample are sequenced and compared. In this way an accuracy of at least 99.99% per base is achieved.

 
Non-assembled
Assembled
SS
DS
SS
DS
Fast sequencing with 1 run per day on average
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Fast in-house design and synthesis of primers
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Editing of sequences
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Delivery of synthesized sequencing primers on request
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Delivery of chromatograms for every single reaction
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Accurate assembly of sequences
 
 
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Verification of an assembled sequence with the original sequence
 
 
 

Service Types

 
 
Non-assembled Primer Walking Service (Single-stranded and Double-stranded)
Upon sequencing your template, you will receive the individual sequences. Final assembly will have to be done at your end.
 
Assembled Primer Walking Service (Single-stranded and Double-stranded)
Assembly of the single sequences will be completed quickly and accurately at Microsynth. You will receive all the individual sequences together with an electronic version of the aligned sequence. 
 
Primer Walking Service with Sequence Verification (Double-stranded)
Your sequences will first be assembled at Microsynth and then verified with the original sequence you will provide. Together with the individual sequences you will receive an electronic version of the aligned sequence as well as a report of discrepancies.

How to order

 

Procedure to use our Primer Walking Service for sequencing:
  • Enter our webshop
  • Click on Single Tube Sequencing in the green "DNA Sequencing" area
  • Click on the corresponding Fill Order Form under "Primer Walking" and follow the further instructions