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Reference Transcriptome Sequencing
Conduct a differential gene expression analysis when there is no reference genome
Discover differential gene expression where no one has looked before
Considerations before starting a reference transcriptome sequencing project:
- Is there a „nearby“ reference genome?
- Deep sequencing or cDNA normalization?
- Aim of the study versus limits of a reference transcriptome?
- Short term goals and long term goals?
- Initial setup (expression states)?
- Functional setup (replicates and conditions)?
Let us guide you – from design to analysis
Example projects using reference transcriptome sequencing:
- Functional expression study on an organism with vague taxonomy
- Breeding studies and selection
- Gene expression for interesting/selected phenotypes
Applications related to reference transcriptome sequencing:
- RNA sequencing
- small RNA sequencing
Our analysis module provides de novo information on previously unknown transcriptomes. The results provided include a broad overview of the transcriptome, the transcript sequences and their detailed annotation.
- Information on the GC content and size distribution of the assembled transcripts. (see Figure 1)
- Information on the exact transcript sequences. (see Table 1)
- Annotation of the transcript sequences and links to available information found in public databases. (see Table 2)
Figure 1: Histogram of transcipt sizes and their GC content.
Table 1: This detail of a result table lists two exemplary transcripts.
Table 2: Detail of a results table listing parts of the annotation for the two exemplary transcripts.