Sample Requirements

Please take some time to review our sample requirements. By following these guidelines you increase the quality of your results and you give us the possibility to help you further.

Concentration

Template	Concentration
Plasmid	40-100 ng/µl
PCR (purified and unpurified products)* PCR 1000 - 2000 bp PCR 500 - 1000 bp PCR 250 - 500 bp PCR 100 - 250 bp	General Rule: 1.5 ng/µl per 100 bp 30 ng/µl 15 ng/µl 7.5 ng/µl 4 ng/µl
E.coli	50 µl LB Medium OR visible colony on agar plate
E.coli (Ecoli NightSeq®)	add 1 colony (diameter 1 mm) OR add 5 µl turbid liquid culture
Plasmid (Full PlasmidSeq)	20 ng/µl
Genomic DNA (BacterialSeq)	50 ng/µl

*Upfront PCR purification is highly recommended but can easily be outsourced to Microsynth.

Primer	Concentration
Premixed	4 pmol/µl = 4 µM
As separate tube (enclosed)	10 pmol/µl = 10 µM

Volumes

Service	Volume	Additional Information
Economy Run (Tube)	12 µl	Premixed option: 12 µl DNA template solution + 3 µl 20µM sequencing primer solution
Economy Run (Plate)	10 µl/well in 96-well plate	Increase the volume by 5 µl /well for each additional reaction
Premium Run	At least 20 µl	Increase the volume by 5 µl for each additional reaction
Primer Walking Service	At least 20 µl	Single Stranded 0.5 µg of plasmid-DNA for every kb of your construct Double stranded 1 µg of plasmid-DNA for every kb of your construct
Full PlasmidSeq	At least 15 µl	Suitable plasmid sizes range from 2.5 kb to 25 kb. While plasmids ranging from 25 kB to 50 kb have not been tested yet, you may try them at your own risk.
BacterialSeq	At least 20 µl	High Quality DNA is needed for this services. That means high-molecular weight DNA with no fragments <5 kb.

Primer	Volume
As separate tube (enclosed)	20 µl + 3 µl per additional reaction

Storage

Service	Storage at Microsynth
Economy Run (Tube)	4 days
Economy Run (Plate)	1 month
Ecoli NightSeq® (Tube or Plate)	1 month
Premium Run	3 months
Primer Walking Service	3 months
Primer (stored)	4 months
Primer (custom primer list)	10 months