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STR and RFLP Analysis
Complete services for short tandem repeat (STR) and restriction fragment length polymorphism (RFLP) analysis. Whether your application is a plain routine service or more complex R&D, Microsynth can offer you both the know-how and the necessary lab infrastructure.
Features and Benefits
- PCR amplification of STR loci and separation on state-of-the art capillary electrophoresis systems (e.g. ABI 3730xl)
- Allele size and identity determination with GeneMarker software
- Optimization of PCR amplification conditions (e.g. for detecting STRs)
- Design and synthesis of high quality fluorescent-labeled PCR primers for individual or multiplex strategies via our in-house oligonucleotide production facility
- Data presented in user-friendly spreadsheets that contain fragment length/allele size and other parameters of interest
- Data or main results are made available in electronic or hard copy formats; additional information such as electropherogram for publication matters can be made available upon request
- A "ready-to-load" service is available on our capillary electrophoresis system if the user has prepared the fluorescently labeled PCR products in 96-well plates
- Experience with difficult-to-assay samples and complete assistance with data interpretation
Some of the applications for STR and RFLP analysis include:
DNA fingerprinting. Forensic scientists may use it identify suspects based on evidence samples collected at scenes of crimes.
Paternity testing. STR and RFLP are also used in the determination of paternity or for tracing ancestry.
Genetic diversity. The techniques can be used in studying evolution and migration of wildlife, studying breeding patterns in animal populations and in the detection and diagnosis of certain diseases.
How to Order
Every genotyping project is different. Please ask our application specialists for your quote.
The following questions are relevant for appropriate pricing:
- DNA/RNA isolation necessary? From what kind of organism and tissue?
- Expected number of samples (single analysis, repeated analysis)?
- Delivery of samples (amount, quality and vessel)?
- Assay established?
- Single or multiplex assay?
- Number of replicas per sample?
- Delivery form of data (raw data, analysed data, report)
- Test samples available? Especially in case of larger projects, we prefer to perform initial testing in order to make a good bid.